第一章 单元测试

1、判断题:
At the isoelectric point, the net charge of the protein molecule is 0.
选项:
A:错
B:对
答案: 【

2、判断题:
All the proteins have quaternary structure.
选项:
A:错
B:对
答案: 【

3、单选题:
The principle of salt precipitation, precipitation of proteins is ( )
选项:
A:Lowering the dielectric constant of the protein solution
B:Binding of proteins to form insoluble protein salt
C:Adjust the isoelectric point of the protein solution
D:To neutralize the charge and destroy the hydration shell
E:Denature proteins
答案: 【To neutralize the charge and destroy the hydration shell

4、单选题:
The main chemical bond that maintains the alpha-helical structure of the protein is( )
选项:
A:Hydrogen bonding between peptide bond atoms
B:Hydrogen bonding between side chains
C:peptide bond
D:Salt key
E:Disulfide bond
答案: 【Hydrogen bonding between peptide bond atoms

5、单选题:
The advanced structure of the protein depends on ( )
选项:
A:Amino acid composition and sequence in protein peptide chains
B:Peptide units in protein peptide chains
C:Peptide bond plane in protein peptide chain
D:Peptide bonds in protein peptide chains
E:Hydrogen bonds in protein peptide chains
答案: 【Amino acid composition and sequence in protein peptide chains

6、单选题:
The physical and chemical properties shared by proteins and amino acids are: ( )
选项:
A:Amphoteric Dissociation
B:Colloidal properties
C:Polymer properties
D:Precipitation properties
E:Denatured properties
答案: 【Amphoteric Dissociation

7、多选题:
The properties of denatured proteins are ( )
选项:
A:Loss of biological activity
B:precipitation
C:Solubility drops significantly
D:Easy to be hydrolyzed by protease
E:Solidification
答案: 【Loss of biological activity;
Solubility drops significantly;
Easy to be hydrolyzed by protease

8、多选题:
Protein denaturation is because ( )
选项:
A:The hydration membrane is destroyed and the charge is neutralized
B:Peptide bond cleavage
C:Subunit depolymerization
D:Hydrogen bond is destroyed
E:Disulfide bond cleavage
答案: 【Subunit depolymerization;
Hydrogen bond is destroyed;
Disulfide bond cleavage

9、多选题:
Primary structure of protein ( )
选项:
A:It mainly depends on peptide bond to maintain stability
B:Is the basis of spatial structure
C:refers to amino acid sequence
D:It does not include disulfide bond
E:α-helix and β-sheet are the major conformations of it
答案: 【It mainly depends on peptide bond to maintain stability;
Is the basis of spatial structure;
refers to amino acid sequence

10、多选题:
Which of the following are acid amino acids? ( )
选项:
A:Aspartate
B:Lysine
C:Alanine
D:Arginine
E:Glutamate
答案: 【Aspartate;
Glutamate

第二章 单元测试

1、判断题:
Tm is directly related to the GC content of DNA and ionic strength.
选项:
A:错
B:对
答案: 【

2、判断题:
rRNA is the site for protein biosynthesis.
选项:
A:错
B:对
答案: 【

3、单选题:
In a DNA molecule, if the molar ratio of A is 17.2%, the molar ratio of C should be ( )
选项:
A:65.6%
B:17.2%
C:67.2%
D:32.8%
E:50.3%
答案: 【32.8%

4、单选题:
The key part of DNA molecule to store and transmit genetic information is ( )
选项:
A:base sequence
B:pentose phosphate skeleton
C:base rotation angle
D:Pentose conformation
E:phosphate diester bond
答案: 【base sequence

5、单选题:
The force to maintain the lateral stability of DNA double helix is ( )
选项:
A:disulfide bond
B:phosphodiester bond
C:Base packing force
D:Internal screw drainage
E:hydrogen bond between base pairs
答案: 【hydrogen bond between base pairs

6、单选题:
What's wrong with nucleic acid hybridization ( )
选项:
A:DNA single strand can hybridize with RNA strand with almost the same complementary base
B:RNA DNA hybrid chain can be synthesized by reverse transcription using mRNA as template
C:Using molecular hybridization, the target gene can be fished from gene library
D:mRNA can be hybridized with the encoded polypeptide chain
E:Two single stranded DNA from different sources can be hybridized as long as some bases are complementary
答案: 【mRNA can be hybridized with the encoded polypeptide chain

7、多选题:
The changes in DNA denaturation are: ( )
选项:
A:covalent bond fracture
B:Viscosity increase
C:Hydrogen chain breaking and double helix structure breaking between chains
D:hyperchromic effect
答案: 【Hydrogen chain breaking and double helix structure breaking between chains;
hyperchromic effect

8、多选题:
The histones that make up the core particle of nucleosome are ( )
选项:
A:H2A
B:H4
C:H1
D:H2B
E:H3
答案: 【H2A;
H4;
H2B;
H3

9、多选题:
As for the description of rRNA, it is correct to ( )
选项:
A:Containing more rare bases
B:Large and small subunits that can combine with proteins to form ribosomes
C:No secondary structure
D:Shortest half-life
E:It's the most abundant RNA in the cell
答案: 【Large and small subunits that can combine with proteins to form ribosomes;
It's the most abundant RNA in the cell

10、多选题:
The biological functions of nucleotides in vivo include ( )
选项:
A:Raw materials for nucleic acid synthesis in vivo
B:As cytoskeleton
C:Some nucleotides can be used as second messengers of hormones
D:Some nucleotides are the direct energy suppliers in the organism
E:Some nucleotides are allosteric effectors of allosteric enzymes
答案: 【Raw materials for nucleic acid synthesis in vivo;
Some nucleotides can be used as second messengers of hormones;
Some nucleotides are the direct energy suppliers in the organism;
Some nucleotides are allosteric effectors of allosteric enzymes

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